Angiogenesis 5: 275–354, 2002.
4th Symposium on the Biology of Endothelial Cells Molecular Cell Biology of the Endothelium in Development and Diseases July, 18–20, 2003, Munich, Germany Organized by Prof. Dr. Michael Stu¨rzl, GSF National Research Center for Environment and Health Location: Ho¨rsaal des Lehrstuhls Physiologie (LMU), Pettenkoferstrasse 12
Conference Program Friday, July 18, 2003 09.00–12.30
09.00
Introductory Remarks: Ulrich Pohl (Mu¨nchen, D)
Workshop: Intravital Microscopy and Live Cell Imaging in Angiogenesis Research (Chair: U. Pohl)
09.10
Steffen-Sebastian Bolz (Mu¨nchen, D), L1 Application of optical and transfection techniques from cell physiology to intact resistance arteries
09.40
lan MacDonald (London, C), L2 Intravital videomicroscopy in angiogenesis research
10.10
Peter Vaikoczy (Mannheim, D), L3 Intravital fluorescence videomicroscopy to study tumor angiogenesis and microcirculation
10.40
Discussion and Coffee
11.05
Arjan W. Griffioen (Maastricht, NL), L4 Intravital microscopy to study leukocyte-vessel wall interactions in tumor microvasculature
11.35
Rainer Pepperkok (Heidelberg, D), L5 Studying vesicular coat assembly and membrane interactions in living cells with sub-resolution microscopy techniques
12.05
Discussion
12.30–14.00 13.45–14.00
Lunch Break & Opening Industrial Exhibition Welcoming Remarks: Michael Stu¨rzl, GSF National Research Center for Environment and Health, Neuherberg, Germany Ernst-Guenter Afting/Ju¨rgen Kinder, GSF National Research Center for Environment and Health, Neuherberg, Germany Ulrich Pohl, Institut of Physiology, LMU, Munich, Germany
276
Conference program
14.00–16.30
Invited Lectures:
Endothelial Progenitor Cells (Chair: A. Hatzopoulos)
14.00
Ju¨rgen Hescheler (Ko¨ln, D), L6 Transgenic embryonic stem cells in cardiovascular research
14.45
Christian Beltinger (Ulm, D), L7 Endothelial progenitors: vehicles for tumor therapy
15.15
Cornell Badorff (Frankfurt, D), L8 Differentiation of human adult endothelial progenitor cells into functionally active cardiomyocytes Lectures:
16.30–17.00
15.45
Jan Horstkotte (Mu¨nchen, D), L9 Role of embryonal EPCs in attenuating postischemic reperfusion injury in mice hearts
16.00
Hetty de Boer (Utrecht, NL), L10 Impaired differentiation and adhesion of endothelial progenitor cells under diabetic conditions
16.15
Anita Jo¨rg (Neuherberg, D), L11 Impaired expression of MMP-1 in endothelial precursor cells and HUVEC of infants born to HIV-1-infected women
Coffee Break
17.00–19.30
Invited Lectures:
The Endothelium in Development and Angiogenesis I (Chair: B. Bader)
17.00
Georg Breier (Bad Nauheim, D), L12 Role of VEGF and endothelial HIFs during embryonic angiogenesis
17.25
Kari Alitalo (Helsinki, SF), L13 Growth factors and gene programs for angiogenesis vs. lymphangiogenesis
18.05
Michael Detmar (Charlestown, USA), L14 Angiogenesis and lymphangiogenesis progression and inflammation
in
tumor
Lectures:
20.00–22.00
18.45
Amparo Palmer (Martinsried, D), L15 Protein complexes transducing ephrin B reverse signaling
19.00
Valentin Djonov (Bern, CH), L16 VEGF withdrawal induces vascular remodeling and pruning via intussusception
19.15
Torsten Gloe (Mu¨nchen, D), L17 Shear stress induced elastase-fragmentation of laminin to E8 prevents endothelial cell proliferation and differentiation
Poster Session and Warm-up Party
Conference program
277
Saturday, July 19, 2003 Invited Lectures:
08.30–10.20 The Endothelium in Development and Angiogenesis II (Chair: M. Gawaz)
08.30
Hellmut Augustin (Freiburg, D), L18 Molecular analysis of the angiopoietin/Tie-2 and Ephrin/Eph ligand receptor systems during angiogenesis
08.55
Jacques Pouysse´gur (Nizza, F), L19 Hypoxia signaling and angiogenesis: in vivo interplay between oxygen sensors
09.35
lan MacDonald (London, C), L20 Intravital fluorescence microscopy of microvascular development in metastases Lectures:
10.20–10.50
10.00
Horst-Dietmar Muller (Wien, A), L21 Specific signals of VEGFR-2 via PKC/EGR-1 and calcineurin/NFAT lead to angiogenic gene induction in endothelial cells
10.10
Ruth Michaelis (Frankfurt, D), L22 Cytochrome P450 2C9 induces cyclo-oxygenase-2 (Cox-2) expression in endothelial cells
Coffee Break
10.50–12.30 The Endothelium in Atherosclerosis (Chair: W. Erl, W. Siess, P.C. Weber)
Invited Lecture: 10.50
Norbert Leitinger (Wien, A), L23 Inflammation induced by oxidized lipids: a role in atherosclerosis Lectures:
11.20
Victoria Kolb-Bachofen (Du¨sseldorf, D), L24 The critical role of L-arginine in endothelial cell survival during oxidative stress
11.30
Regine Heller (Jena, D), L25 Alpha-tocopherol and its water-soluble derivative trolox potentiate endothelial NO synthesis via different mechanisms Invited Lecture:
11.40
Christian Weber (Aachen, D), L26 Chemokines and adhesion molecules in atherogenic monocyte recruitment Lecture:
12.10
Felicitas Besta (Mu¨nchen, D), L27 Enhanced uPAR expression in atherosclerotic lesions of APO E-deficient mice is associated with a decrease in b3-endonexin and enhanced NF-jB acitivity
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Conference program
Lecture: 12.20
12.30–14.00
Steffen Maßberg (Mu¨nchen, D), L28 Role of platelet-endothelial cell interactions for atherosclerosis
Lunch Break
14.00–15.45
Invited Lectures:
The Endothelium in Inflammation (Chair: M. Stu¨rzl)
14.00
Dietmar Vestweber (Mu¨nster, D), L29 Molecular mechanisms involved in the control of interendothelial cell contacts
14.40
Meinrad Gawaz (Mu¨nchen, D), L30 Platelet adhesion to the endothelium: role for inflammation and microcirculation Lectures:
15.45–16.15
15.00
Clara Lubeseder-Martellato (Neuherberg, D), L31 The guanylate binding protein-1 is a tissue and serum marker of inflammatory cytokine activated endothelial cells
15.10
Eric Guenzi (Neuherberg, D), L32 The GTPase function of GBP-1 controls invasiveness and angiogenic capability of endothelial cells through the inhibition of MMP-1 expression
15.25
Stefan W. Schneider (Mu¨nster, D), L33 Imaging of haemostatic active von Willebrand factor multimers on the apical surface of human endothelial cells
15.35
Marion Haubitz (Hannover, D), L34 Circulating endothelial cells in bone marrow transplanted patients – a marker of endothelial toxicity?
Coffee Break
16.15–19.10 The Endothelium as a Target of Therapy (Chair: C. Kupatt)
Invited Lectures: 16.15
Robert Kerbel (Toronto, C), L35 Cellular and molecular basis for the selective antiangiogenic effects of frequent low-dose (metronomic) chemotherapy
16.55
Wadih Arap (Houston, USA) + Renata Pasqualini (Houston, USA) (joint lecture), L36 tba
17.40
Markus Essler (Mu¨nchen, D), L37 Targeting tumor vasculature using specific homing peptides: pitfalls and prospects
18.00
Arjan Griffioen (Maastricht, NL), L38 Development of novel angiogenesis inhibitors
Conference program
279
Lectures: 18.20
Karl-Heinz Thierauch (Berlin, D), L39 Concept-based discovery and profiling of PTK787/ ZK222584, a novel and orally active angiogenesis inhibitor entering phase III studies
18.40
Rabea Hinkel (Mu¨nchen, D), L40 Hsp90 transfection reduces ischemia/reperfusion-induced myocardial dysfunction via reciprocal eNOS Ser 1177 phosphorylation and Thr 495 dephosphorylation
18.50
Jozef Dulak (Krakow, PL), L41 Overexpression of CuZn superoxide dismutase enhances the synthesis of vascular endothelial growth factor
19.00
Christiane Bruns (Mu¨nchen, D), L42 Anti-angiogenic and anti-tumor activity of a novel VEGFR-2-tyrosine kinase inhibitor ZD6474 in a metastatic human pancreatic tumor model
19.10–19.30
Poster Awards
20.00
Bavarian Evening in the Augustiner Keller
Sunday, July 20, 2003 09.00–10.35
Invited Lectures:
The Endothelium in Infectious Diseases (Chair: M. Aepfelbacher)
09.00
09.25
Norbert Leitinger (Wien, A), L43 Oxidized phospholipids inhibit properties of LPS
immunostimulatory
Paolo Monini (Rom, I), L44 Inhibition of Kaposi’s sarcoma (KS) by blocking endothelial cell and KS cell invasion with HIV protease inhibitors Lectures:
10.35–10.50
10.05
Christian Sinzger (Tu¨bingen, D), L45 The cell tropism of human cytomegalovirus (HCMV) depends on the efficiency of a nocodazol-sensitive transport of penetrated virus particles towards the cell nucleus
10.20
Walter Da¨ubner (Du¨sseldorf, D), L46 Restriction of herpes simplex virus type 1 replication in human brain microvascular endothelial cells: role of indolamine 2,3 dioxygenase
Coffee Break Invited Lecture: 10.50
Klaus Preissner (Giessen, D), L47 Changing partners in leukocyte recruitment: new adhesion molecules and their coupling during inflammation
280
Conference program Lectures:
12.00
End/Departure
11.30
Andreas Schro¨der (Mu¨nchen, D), L48 Invasion and survival of staphylococcus aureus in endothelial cells
11.45
Alexandra Unkmeier (Wu¨rzburg, D), L49 Neisseria meningitidis-induced inflammatory cytokine release from human brain endothelial cells involves stress response kinases
